Waters Oasis Mixed-Mode Ion-Exchange Cartridges and 96-We Manuale Utente Scaricare il pdf

CONTENTS

I. INTRODUCTION

II. SAMPLE PRE-TREATMENT

a. Biological Samples

b. Solid Samples: Soil, Whole foods, Tissue

c. Aqueous Samples: Water, Beverages

d. Non-Aqueous Liquid

III. SOLID-PHASE EXTRACTION PROCEDURE

FOR BASIC COMPOUNDS USING OASIS

MCX AND STRONGLY ACIDIC COMPOUNDS

USING OASIS WAX

IV. SOLID-PHASE EXTRACTION PROCEDURE

FOR ACIDIC COMPOUNDS USING OASIS

MAX AND STRONGLY BASIC COMPOUNDS

USING OASIS WCX

V. OASIS SORBENT SELECTION AND

PROTOCOL CHART

VI. OASIS 2X4 METHOD OPTIMIZED FOR

�ELUTION PLATE

VII. OASIS PST �ELUTION METHOD OPTIMIZED

FOR THERAPEUTIC PEPTIDES BIOANALYSIS

VIII. OASIS 96 WELL PLATES

IX. ORDERING INFORMATION

*The COA, contained in the Oasis Package, displays

results from stringent quality control tests on the batch

of copolymer sorbent and the lot of packed plates.

I. INTRODUCTION

The Oasis

family of solid-phase extraction (SPE) products

are designed to simplify and improve your sample preparation

by combining the right sorbent chemistry, device format and

methodology. There are five available Oasis Sorbent Chemistries

which are designed to meet all of your sample preparation needs.

They are all built upon a unique, water-wettable Oasis HLB

(Hydrophilic-Lipophilic Balance) copolymer and provide exceptional

results. The sulfonic acid MCX (Mixed-Mode Cation-eXchange),

and quaternary amine MAX (Mixed- Mode Anion-eXchange)

derivatives of Oasis HLB provide dual modes of retention (e.g.

both reversed-phase and ion-exchange retention modes available),

enabling greater cleanup selectivity and sensitivity for basic and

acidic compounds respectively— even if the sorbent in the wells

runs dry. The carboxylic acid WCX (Weak Cation-eXchange) and

the piperazine WAX (Weak Anion eXchange) are also derivatives

of Oasis HLB and provide dual modes of retention. These sorbents

are specifically designed to offer the same benefits and features

as HLB with the ability to retain and release strong bases (e.g.

quaternary amines) and strong acids (e.g. sulfonates) respectively.

All of the five patented Oasis Chemistries are available in several

device formats (e.g. cartridges, 96-well plates, and μElution plates)

to fit your specific needs.

Note: Because the Oasis HLB Sorbent wets as well with water as with

methanol, there is no need to keep the sorbent in the wells wet prior

to sample loading. This benefit not only saves you time but provides

higher and more reproducible recoveries than those obtained on

traditional sorbents.

OASIS MIXED-MODE ION-EXCHANGE CARTRIDGES AND 96-WELL PLATES

[ CARE AND USE MANUAL ]

1 2 3 4 5 6

[ CARE AND USE MANUAL ] 1

Sommario

Pagina 1 - [ CARE AND USE MANUAL ]

CONTENTSI. INTRODUCTIONII. SAMPLE PRE-TREATMENT a. Biological Samples b. Solid Samples: Soil, Whole foods, Tissue c. Aqueous Samples: Water, Be

Pagina 2

II. SAMPLE PRE-TREATMENTa. Biological SamplesThis section contains recommendations for preparing your biological samples (plasma, serum, urine, etc.)

Pagina 3

15. Switch off the vacuum pump or stop vacuum by closing the valve. (before switching off the vacuum pump, please reduce the vacuum to the lowe

Pagina 4

Table 1: Recommended Volume for Generic Methods (assuming 1:1 dilution)Cartridges 96-well plate µElution PlateCartridges size/Sorbent mass1 cc 3 cc 6

Pagina 5

VI. OASIS 2X4 METHOD OPTIMIZED FOR µELUTION PLATEThe proven Oasis 2x4 Method elution solvent is optimized to accommodate the elutropic requiremen

Pagina 6

IX. ORDERING INFORMATION Description Part NumberUPLC PST Therapeutics Peptide Method Development Kit176001835HPLC PST Therapeutics Peptide Method Deve

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