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[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. STANDARD PREPARATION

III. ADDITIONAL RESOURCES

IV. ORDERING INFORMATION

Biopharmaceutical Training Kit

I. INTRODUCTION

The Biopharmaceutical Training Kit is designed to provide trainers

and customers with high quality standards that can be utilized as a

starting point for running biopharmaceutical applications on their

Waters UPLC inlet with Tof or Q-Tof mass spectrometry detection

systems. The training kit pulls together many of the individual

standard samples that are often used in demos and training classes,

including reference compounds for calibration and lockmass. This

kit provides a foundation for biopharmaceutical LC-MS analysis and

can be used for optimization of methods prior to running valuable

samples to ensure system performance and confidence in results.

Table 1. Kit Components for Biopharmaceutical Training Standards (P/N 186006921)

Part # Contents Description of Components Acid

Amount

per Vial

Container

186006921-1 1 x 15 mL Horse Heart Myoglobin 2.5 mg 15 mL Clear Nalgene Bottle

186006921-2 1 vial Intact mAb Mass Check Standard 1 mg

TruView™ Max Recovery with non-slit PTFE/

silicone Septa (p/n 186005668CV)

186006921-3 1 vial Glu-fibrinopeptide B 0.5 mg 4 mL Amber Screw Top Vial

186006921-4 1 vial MassPREP Enolase Digest 1 nmol

TruView Max Recovery with non-slit PTFE/

silicone Septa (p/n 186005668CV)

186006921-5 1 vial Reverse Peptide Ser-Asp-Gly-Arg-Gly 1.0 mg of each 4 mL glass amber bottle with screw top

186006921-6 1 vial Reverse Peptide Gly-Arg-Gly-Asp-Ser 1.0 mg of each 4 mL glass amber bottle with screw top

Table 2. Kit Components for Biopharmaceutical Prep Kit (P/N 186007517)

Part # Contents Description of Components Acid

Amount

per Vial

Container

186007517-1 1 x 2 mL Formic Acid 2 mL 2 mL Ampoule

186007517-2 1 x 30 mL Sodium Cesium Iodide 2 μg/μL 30 mL Nalgene

290002010 3 30 mL Nalgene Fludics Bottle (with cap) Empty 30 mL Nalgene Fludics Bottle (with cap)

The Biopharmaceutical Training Kit comes in two separate boxes. The Biopharmaceutical Training Standard Kit (see Table 1) contains

the standards. This kit is shipped at ambient temperatures, however upon receipt of this kit, please store at -20 °C to maximize long

term stability. The Biopharmaceutical Training Prep Kit (see Table 2) contains the materials needed for solubilization and standard prep.

This kit is shipped at ambient temperatures and can be stored at room temperature. Once any component is opened, use right away for

optimum results.

1 2 3 4 5

[ CARE AND USE MANUAL ] 1

Sommario

Pagina 1 - [ CARE AND USE MANUAL ]

[ CARE AND USE MANUAL ]CONTENTS I. INTRODUCTION II. STANDARD PREPARATION III. ADDITIONAL RESOURCES IV. ORDERING INFORMATION Biopharmaceutica

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2[ CARE AND USE MANUAL ]Biopharmaceutical Training KitII. STANDARD PREPARATIONSection 1. Diluent Preparationa. 90:10 water:acetonitrile + 0.1% formic

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3[ CARE AND USE MANUAL ]Biopharmaceutical Training KitSection 2. Sample Preparationa. Sodium Cesium Iodide: 186007517-2 1 x 30 mLSodium Cesium Iodide

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4[ CARE AND USE MANUAL ]Biopharmaceutical Training Kit2.5 pmol/µL Optional Analyte Enolase digest (Comparative) sample preparation: 1. Using a 200 µ

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]Waters, The Scien

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