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[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. STORAGE & STABILITY

III. PREPARING THE FORENSIC TOXICOLOGY

INSTALLATION STANDARD

IV. BUFFER PREPARATION

V. EXAMPLE USING THE TOXICOLOGY

INSTALLATION STANDARD

VI. ORDERING INFORMATION

VII. ADDITIONAL RESOURCES

FORENSIC TOXICOLOGY INSTALLATION STANDARDS KIT

I. INTRODUCTION

The Waters Forensic Toxicology Installation Standards Kit (Part

Number 186007361) is intended for use as a qualitative retention

time standard for LC-MS applications using positive ion electrospray.

The kit contains three components:

1. Forensic Toxicology Installation Standard (186007361-1)

The components and concentrations in this analytical standard

can be seen in Table 1. The standard is shipped in a crimp

top vial containing 1.5 mL of solution in a matrix of 5 mM

ammonium formate at pH 3.00.

Table 1. Component Concentrations

Analyte Concentration

Nicotine 0.5 µg/mL

Scopolamine 0.5 µg/mL

Milnacipran 0.5 µg/mL

Triprolidine 0.5 µg/mL

Tianeptine 0.5 µg/mL

Tiapride 0.5 µg/mL

Buflomedil 0.5 µg/mL

Trazodone 0.5 µg/mL

Clozapine 0.5 µg/mL

Perphenazine 0.5 µg/mL

2. Ammonium Formate Concentrate (186007361-2)

A concentrated solution of ammonium formate which makes a

pH controlled buffer solution intended for use as mobile phase

for LC (see IV. Buffer Preparation). Diluted as specified, the final

solution is 5mM ammonium formate at pH 3.0. This solution is

shipped in a 15 mL brown Nalgene

bottle containing 10 mL of

concentrate in a matrix of water with 9.75 % formic acid.

3. Formic Acid (186007361-3)

Formic acid is used to adjust the pH of the ammonium formate

buffer (see IV. Buffer Preparation). Shipped in a 2 mL amber

flame sealed ampoule containing 1mL of ≥ 98% formic acid and

once opened store any unused acid in the supplied Waters LCMS

Certified Screw Top Vial (186005668CV).

1 2 3

[ CARE AND USE MANUAL ] 1

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[ CARE AND USE MANUAL ]CONTENTSI. INTRODUCTIONII. STORAGE & STABILITYIII. PREPARING THE FORENSIC TOXICOLOGY INSTALLATION STANDARDIV. BUFF

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2[ CARE AND USE MANUAL ]Forensic Toxicology Installation Standards KitII. STORAGE AND STABILITYThe Waters Forensic Toxicology Installation Standards K

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]Waters, The Scien

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