Waters iKey Manuale Utente

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[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. iKEY FEATURES
a. Analytical iKey
b. Accessory iKeys
c. iKey Component Description
III. CONNECTING THE iKEY TO THE
ionKEY SOURCE
IV. iKEY USAGE
a. Sample Preparation
b. Mobile Phases
c. pH Range
d. Pressure
e. Temperature
V. REMOVING THE iKEY FROM THE
ionKEY/MS SYSTEM
VI. CARING FOR THE iKEY
a. Cleaning and Regeneration
b. Storage
c. Good Practices
VII. TROUBLESHOOTING iKEY PERFORMANCE
VIII. ORDERING INFORMATION
iKey Separation Device
I. INTRODUCTION
The Waters iKey™ Separation Device is a high-pressure,
microfluidic device that provides UPLC
®
separations with
the ionKey/MS™ System. The iKey eliminates manual fitting
connections and minimizes the potential for manual variability
and extra-column dispersion.
The iKey contains a ceramic substrate produced using a modified,
co-fired ceramic manufacturing process which contains an
analytical separation channel packed with stationary phase, an
electrospray emitter, a column heater, and an integrated memory
device. The integrated memory maintains the number of injections,
maximum backpressure, and number of sample sets run. A circuit
in the iKey senses and controls temperature as well.
The iKey is manufactured to exacting specifications, providing
outstanding peak symmetry for maximum sensitivity and
accurate quantitation. Each iKey is individually tested to
ensure that it passes stringent quality control requirements.
Compared to conventional chromatographic systems, successful
day-to-day performance of the ionKey/MS System requires
certain considerations. This document provides several essential
recommendations for the successful use of the iKey. The iKey
is the only analytical consumable device compatible with
ionKey/MS System.
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Sommario

Pagina 1 - [ CARE AND USE MANUAL ]

[ CARE AND USE MANUAL ]CONTENTSI. INTRODUCTIONII. iKEY FEATURES a. Analytical iKey b. Accessory iKeys c. iKey Component DescriptionIII. CON

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10[ CARE AND USE MANUAL ]iKeyDon’t assume the iKey is to blame: Investigate cause of iKey failure; Monitor backpressure; Examine mobile phase

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]Waters, The Scien

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2[ CARE AND USE MANUAL ]iKeyII. iKEY FEATURESa. Analytical iKeyThis iKey has a 150 µm x 50 mm or 150 μm x 100 mm analytical separation channel availab

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3[ CARE AND USE MANUAL ]iKeyb. Accessory iKeysFlow Injection iKey: This iKey has no packing material. It enables one to inject a sample from the sampl

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4[ CARE AND USE MANUAL ]iKeyiKeySTOP FLOWSTATUS PRESSUREIII. CONNECTING THE iKEY TO THE ionKEY SOURCEThese procedures outline the steps necessary to c

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5[ CARE AND USE MANUAL ]iKeyIV. iKEY USAGETo ensure the continued high performance of your iKey Separation Device, please follow these guidelines: a.

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6[ CARE AND USE MANUAL ]iKey5) Consider filtering samples with a 0.2 µm membrane to remove particulates. If the sample is dissolved in a solvent that

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7[ CARE AND USE MANUAL ]iKeyb. Mobile PhasesTo maintain and ensure maximum iKey performance, use high quality chromatography solvents. Use only filter

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8[ CARE AND USE MANUAL ]iKeyV. REMOVING THE iKEY FROM THE ionKEY SOURCECAUTION: Opening the locking lever on the ionKey system without first stopping

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9[ CARE AND USE MANUAL ]iKeyb. Storage For periods longer than four days, store the reversed-phase iKey in 100% acetonitrile. Do not store iKeys in bu

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