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CONTENTS

I. INTRODUCTION

II. INSTALLING THE MASSPREP ON-LINE

DESALTING CARTRIDGE INTO THE SENTRY

2.1 X 10 MM GUARD COLUMN HOLDER

III. RECOMMENDED LC/MS SYSTEM CONFIGURATION

TO MINIMIZE MS SOURCE CONTAMINATION

IV. SUGGESTED METHOD FOR MASS ANALYSIS

USING MASSPREP ON-LINE DESALTING

CARTRIDGE

a. Instrumentation

b. Separation Method

V. GUIDELINES FOR USE AND

TROUBLESHOOTING

VI. MISCELLANEOUS

I. INTRODUCTION

Mass spectrometry is a powerful tool for intact protein analysis.

However, the quality of analyses can be compromised by salts in

the sample that interfere with ionization and/or form adducts with

the investigated proteins. It is therefore important to remove or

significantly minimize the introduction of extraneous salt ions

into the mass analyzer. The use of on-line, desalting devices can

efficiently perform this function with minimal sample handling.

Waters MassPREP™ On-line Desalting Cartridges (2.1 x 10 mm,

P/N 186002785) contain a polymer-based, reversed-phase

packing material that can be used to effectively desalt protein

samples prior to mass spectrometry. (Note: The purchase and use

of Waters Sentry™ 2.1 x 10 mm Guard Column Holder Kit (P/N

WAT097958) is required for this application as shown in Figure 1.)

The phenyl phase material contained in these on-line devices

successfully “traps” proteins allowing the salts to be washed

to waste prior to elution of the desalted protein into the mass

spectrometer. With an optimized LC/MS method, cycle times as low

as 3 minutes are achievable. These on-line devices, when used with

an appropriate HPLC System injector and injector wash solution,

can be used for the sequential analyses of different samples (See

Figures 2 and 3).

This Care and Use manual provides suggested methods for the

successfully use of this product. For the latest list of MassPREP,

BioSuite™ or NanoEase™ column offerings, go to www.waters.com/

lifesciences.

II. INSTALLING THE MASSPREP ON-LINE

DESALTING CARTRIDGE INTO THE SENTRY

2.1 X 10 MM GUARD COLUMN HOLDER

1. Unscrew the two halves of the Sentry 2.1 x 10 mm Guard

Column Holder.

2. Remove the old MassPREP On-line Desalting Cartridge, if

present.

MassPREP On-Line Desalting Cartridge

[ CARE AND USE MANUAL ]

1 2 3 4 5

[ CARE AND USE MANUAL ] 1

Sommario

Pagina 1 - [ CARE AND USE MANUAL ]

CONTENTS I. INTRODUCTION II. INSTALLING THE MASSPREP ON-LINE DESALTING CARTRIDGE INTO THE SENTRY 2.1 X 10 MM GUARD COLUMN HOLDERIII. RECOMME

Pagina 2

3. Install the new MassPREP On-line Desalting Cartridge, noting the flow arrow direction on the device. This should match the flow arrow direction on

Pagina 3

IV. SUGGESTED METHOD FOR MASS ANALYSIS USING MASSPREP ON-LINE DESALTING CARTRIDGE (Considerations to Minimize Protein Carryover Between Injections):H

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m/z1000 1200 1400 1600 1800 2000 2200 2400 2600 2800 3000 3200 3400 3600 3800%0100%0100%0100%0100Injection 97 blankInjection 98 BSAInjection 99 MAbInj

Pagina 5

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comWaters Corporation, Waters. The Science

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