Waters Protein-Pak Hi Res IEX Columns Manuale Utente Scaricare il pdf

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. CONFIGURING AN ACQUITY SYSTEM FOR

USE IN IEX PROTEIN SEPARATIONS

III. GETTING STARTED

a. eCord Installation

b. Column Connectors

c. Column Installation

d. Column Equilibration

e. Useful Functional Tests for

Benchmarking a New Column

IV. COLUMN SPECIFICATIONS AND USE

a. Specifications

b. Sample Preparation

c. Operating pH

d. Solvents

e. Pressure

f. Flow Rate

g. Temperature

V. TROUBLESHOOTING

VI. COLUMN CLEANING, REGENERATION,

AND STORAGE

a. Cleaning and Regeneration

b. Storage

VI. INTRODUCING ECORD INTELLIGENT

CHIP TECHNOLOGY

a. Introduction

b. Installation

c. Manufacturing Information

d. Column Use Information

VIII. ORDERING INFORMATION

Protein-Pak Hi Res IEX Columns and Standards

I. INTRODUCTION

Thank you for choosing a Protein-Pak™ ion-exchange column.

Waters Protein-Pak Hi Res IEX family of columns were developed

to assist in the UPLC

characterization of recombinant proteins

and monoclonal antibodies found in many of today’s novel

biopharmaceutical therapeutics. The non-porous, high-ligand

density particles overcome some of the high molecular weight

limitations present using traditional porous, IEX particles. In

addition, use of a multi-layered network of ion-exchange groups

(SP, CM, or Q) on non-porous particles assist the effective diffusion

and binding of charged proteins or other biomolecules onto the

particles to deliver relatively high sample loading capacities and

component resolution while minimizing column fouling.

The available ion-exchange chemistries include a strong-anion

and weak- and strong-cation exchangers (Table 1), all of which are

stable, over a wide pH range (3–10), high salt concentrations and

standard pressures (see Column Specifications and Use section) via

use of polymer-based particles. Only when the Protein-Pak Hi Res

IEX column series is combined with the ACQUITY UPLC

system

will the full performance benefits of this column be realized.

Table 1

Abbreviation Ion-Exchange Ligand

SP Sulfopropyl

CM Carboxymethyl

Q Quaternary ammonium

columns

1 2 3 4 5 6 7 8 9

[ CARE AND USE MANUAL ] 1

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Pagina 1 - [ CARE AND USE MANUAL ]

[ CARE AND USE MANUAL ]CONTENTS I. INTRODUCTIONII. CONFIGURING AN ACQUITY SYSTEM FOR USE IN IEX PROTEIN SEPARATIONSIII. GETTING STARTED a. eCor

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2[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and StandardsII. CONFIGURING AN ACQUITY SYSTEM FOR USE IN IEX PROTEIN SEPARATIONSAlthough W

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3[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and Standardsc. Column Installation Note: The flow rates given in the procedure below are for a

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4[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and StandardsProtein-Pak Hi Res CM ColumnAU0.0400.0500.0600.0700.0800.0900.0300.0200.0100.0008L

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5[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and StandardsIV. COLUMN SPECIFICATIONS AND USEa. SpecificationsDescriptionProtein-Pak Hi Res

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6[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and Standardsd. Solvents To maintain maximum column performance, use high quality chromatograph

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7[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and Standardsmade, they likely originate from adsorption to some system component. In that case

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8[ CARE AND USE MANUAL ]Protein-Pak Hi Res IEX Columns and StandardsAt the time of manufacture, tracking and quality control information will be downl

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ CARE AND USE MANUAL ]Waters, The Scien

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