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MetID Standard Mix 1

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

II. STORAGE & STABILITY

III. RECONSTITUTION OF THE STANDARD

SOLUTION FOR TESTING

IV. EXAMPLE OF USING OMEPRAZOLE STANDARD MIX

V. ORDERING INFORMATION

VI. REFERENCES

MetID STANDARD MIX

I. INTRODUCTION

Omeprazole Metabolite Mix is used to test and demonstrate the

Waters UNIFI Application Solution for Metabolite Identification.

The standard was designed to both test and confirm LC and MS

performance but also to test and demonstrate the UNIFI system

solution. Omeprazole is a commonly prescribed medication used to treat

acid reflux causing heartburn (known clinically as gastroesophageal

reflux disease, GERD). When the compound is dosed in vivo in human,

two of the commonly detected metabolites which are formed in

human are 5-hydroxy omeprazole and omeprazole sulfone.

Omeprazole Sulfone

Omeprazole

5-Hydroxy Omeprazole

Figure 1. Structures of Omeprazole, 5-Hydroxy Omeprazole, and Omeprazole Sulfone

Using Waters ACQUITY UPLC

BEH column chemistry, the generic

gradient method can be used to analyze and effectively separate the

standard into its parent and 2 authentic metabolite standard components.

The standard mix stock solution comprises Omeprazole, 5-hydroxy

omeprazole and omeprazole sulfone at concentrations of 10, 1 and

0.1 µg/mL respectively.

II. Storage and Stability.

The lyophilized powder is shipped at ambient temperatures, in brown

amber vials in foil packaging. It is highly recommended that upon

receipt, the standard should be stored frozen and thawed before use.

Once reconstituted the sample should be used immediately for

best results. Omeprazole has been reported to be unstable in the

presence of continuous exposure to UV. Care should be taken to prepare

samples and standard curves out of direct light whenever possible. If

1 2 3

Sommario

Pagina 1 - MetID STANDARD MIX

MetID Standard Mix 1[ CARE AND USE MANUAL ]CONTENTSI. INTRODUCTION II. STORAGE & STABILITY III. RECONSTITUTION OF THE STANDARD SOLUTION FOR TEST

Pagina 2

MetID Standard Mix 2[ CARE AND USE MANUAL ]moderate precautions are taken and the samples, once reconstituted, are stored properly at -10°C (and analy

Pagina 3

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Navigare online o scaricare Manuale Utente per Attrezzatura per l’acqua Waters MetID Small Molecule Standard Mix. Waters MetID Small Molecule Standard Mix User Manual Manuale Utente

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Waters MetID Small Molecule Standard Mix Manuale Utente Pagina 1

Navigare online o scaricare Manuale Utente per Attrezzatura per l’acqua Waters MetID Small Molecule Standard Mix. Waters MetID Small Molecule Standard Mix User Manual Manuale Utente

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