Waters CORTECS 1.6 um Columns Manuale Utente

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CONTENTS
I. GETTING STARTED
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
II. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III. COLUMN CLEANING, REGENERATING
AND STORAGE
a. Cleaning and Regeneration
b. Storage
IV. INTRODUCING eCORD INTELLIGENT
CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
V. ADDITIONAL INFORMATION
a. Tips for Maximizing CORTECS Column Lifetimes
b. Getting Started with CORTECS HILIC Columns
Thank you for choosing a Waters CORTECS™ Column. The
CORTECS packing materials were designed specifically for use
with the Waters ACQUITY UPLC
®
System and are manufactured
in a cGMP, ISO 9001 certified plant using ultra pure reagents.
Each batch of CORTECS material is tested chromatographically with
acidic, basic and neutral analytes and the results are held to narrow
specification ranges to assure excellent, reproducible performance.
Every column is individually tested and a Performance
Chromatogram and Certificate of Batch Analysis are provided on
the eCord
Intelligent Chip.
CORTECS Columns were designed and tested specifically for use on
the ACQUITY UPLC System. CORTECS Columns will exhibit maximum
chromatographic performance and benefits when used on the
holistically-designed ACQUITY UPLC System since they were created
and designed to operate with it.
CORTECS Columns
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Sommario

Pagina 1 - CORTECS Columns

CONTENTSI. GETTING STARTED a. Column Connectors b. Column Installation c. Column Equilibration d. eCord Installation e. Initial Column Effi

Pagina 2 - I. GETTING STARTED

2CORTECS ColumnsI. GETTING STARTEDEach CORTECS Column comes with a Certificate of Analysis and a Performance Test Chromatogram embedded within the eCo

Pagina 3

3CORTECS Columnse. Initial Column Efficiency Determination 1. Perform an efficiency test on the column before using it. This test may consist of: a.

Pagina 4 - II. COLUMN USE

4CORTECS ColumnsTable 2: Buffer Recommendations for Using CORTECS Columns Additive/Buffer pKaBuffer Range Volatility (±1 pH unit)Used for Mass Spec Co

Pagina 5

5CORTECS ColumnsA listing of commonly used buffers and additives is given in Table 2. Additionally, the column lifetime will vary depending upon the o

Pagina 6 - AND STORAGE

6CORTECS ColumnsIII. COLUMN CLEANING, REGENERATING AND STORAGEa. Cleaning and RegenerationChanges in peak shape, peak splitting, shoulders on the

Pagina 7 - TECHNOLOGY

7CORTECS ColumnsIV. INTRODUCING eCORD INTELLIGENT CHIP TECHNOLOGYa. IntroductionThe eCord Intelligent Chip provides the history of a column’s p

Pagina 8 - V. ADDITIONAL INFORMATION

8CORTECS ColumnsV. ADDITIONAL INFORMATIONa. Tips for Maximizing CORTECS Column Lifetimes1. To maximize CORTECS Column lifetime, pay close attention t

Pagina 9

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com©2013 Waters Corporation. Waters, ACQUIT

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