Waters Symmetry Columns Manuale Utente

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[ Care and Use ManUal ]
Symmetry Columns 1
i. introduction
Thank you for choosing a Waters Symmetry
®
c o l u m n . S y m m e t r y
c o l u m n s c o n t i n u e t o s e t t h e s t a n d a r d o f p e r f o r m a n c e f o r
reproducibility. As today’s chemists establish new analytical methods
for the latest pharmaceutical and biopharmaceutical products, the
selection of a reproducible HPLC column is essential. The selected
column needs to provide the same chromatographic results over the
life of the new drug product. The excellent reproducibility of Sym-
metry, SymmetryShield™ and Symmetry300™ columns is a result
of our commitment to maintaining the tightest specifications in the
HPLC column industry.
contents
i. introduction
ii. GettinG started
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
iii. column use
a. Guard Columns
b. Sample Preparation
c. pH Range
d. Solvents
e. Pressure
f. Temperature
iV. scalinG up/down isocratic methods
V. troubleshootinG
Vi. column cleaninG, reGeneratinG and storaGe
a. Cleaning and Regenerating
b. Storage
Vii. connectinG the column to the hplc
a. Column Connectors and System Tubing Considerations
b. Band Spreading Minimization
c. Cartridge Installation
d. Measuring System Bandspreading Volume & System Variance
e. Measuring System Volume
Viii. additional information
a. Use of Narrow-Bore (≤3.0 mm i.d.) Columns
b. Impact of Bandspreading Volume on 2.1 mm i.d. Column Performance
c. Non-Optimized vs. Optimized LC/MS/MS System:
System Modification Recommendations
d. Waters Small Particle Size (3.5 μm) Columns – Fast Chromatography
symmetry columns
Physical Characteristics
Packing Chemistry Particle Size Particle
Shape
Pore
Size
Carbon
Load
End-
capped
Symmetry C
18
C
8
3.5, 5 µm
3.5, 5 µm
Spherical
Spherical
100Å
100Å
19%
12%
Yes
Yes
SymmetryPrep C
18
C
8
7 µm
7 µm
Spherical
Spherical
100Å
100Å
19%
12%
Yes
Yes
SymmetryShield RP18
RP8
3.5, 5, 7 µm
3.5, 5, 7 µm
Spherical
Spherical
100Å
100Å
17%
15%
Yes
Yes
Symmetry300 C
18
C
4
3.5, 5 µm
3.5, 5 µm
Spherical
Spherical
300Å
300Å
8.5%
2.8%
Yes
Yes
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Sommario

Pagina 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]Symmetry Columns 1i. introductionThank you for choosing a Waters Symmetry® c o l u m n . S y m m e t r y

Pagina 2

[ Care and Use ManUal ]Symmetry Columns 10©2008 Waters Corporation. Waters, The Science of W hat’s Possible, Symmetry, SymmetryShi

Pagina 3

[ Care and Use ManUal ]Symmetry Columns 2ii. GettinG startedE a c h S y m m e t r y c o l u m n c o m e s w i t h C e r t i f i c a t e o

Pagina 4

[ Care and Use ManUal ]Symmetry Columns 3b. Sample Preparation1. Sample impurities often contribute to column contamina

Pagina 5

[ Care and Use ManUal ]Symmetry Columns 4d. SolventsTo maintain maximum column performance, use high quality chromatography grade solvents. Filter all

Pagina 6

[ Care and Use ManUal ]Symmetry Columns 5Guard c ol umns ne ed to be re plac ed at re gul ar int erval s, a s determined by

Pagina 7

[ Care and Use ManUal ]Symmetry Columns 6In a proper tubing/column connection (Figure 2), the tubing touches the bottom of the column endfitting, with

Pagina 8

[ Care and Use ManUal ]Symmetry Columns 7c. Cartridge InstallationRefer to the installation illustration in Figure 7. Unscrew the end connectors from

Pagina 9

[ Care and Use ManUal ]Symmetry Columns 8d. Measuring System Bandspreading Volume and System VarianceThis test should be performed on an HPLC system w

Pagina 10

[ Care and Use ManUal ]Symmetry Columns 9Viii. additional informationa. Use of Narrow-bore (<3.0 mm i.d.) ColumnsThis section describes how to mini

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