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E. Coli Digestion Standard 1

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CONTENTS

I. INTRODUCTION

II. VIAL CONTENTS

III. STORAGE AND STABILITY

IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE

WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE

(75 m scale) HPLC

V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE

WATERS PROTEIN EXPRESSION SYSTEM USING MICROSCALE

(300 m scale) HPLC

VI. SAFETY AND MSDS INFORMATION

VII. MASSPREP DIGESTION STANDARD ORDERING INFORMATION

MASSPREP E. COLI DIGESTION STANDARD

I. INTRODUCTION

The MassPREP

™

E.coli digestion standard (MPDS E.coli) is intended

to provide a complex biological matrix for producing samples used

to train new users of the Waters Protein Expression System. MPDS

E. coli was prepared by digesting a purified E. coli cytosolic protein

fraction with sequencing grade trypsin. The MPDS mixture is purified,

and does not contain undigested protein, trypsin, or very hydrophilic

components such as salts.

II. VIAL CONTENTS

Each vial contains 100 µg of lyophilized E. coli cytosolic protein

digest.

III. STORAGE AND STABILITY

The lyophilized powder is stable at room temperature until the

expiration date printed on the label, or one year from purchase. Once

reconstituted in 0.1% formic acid, the solution is stable for one week

when stored at 4 °C. Stability can be extended by freezing samples at

-20 °C.

Note: The contamination of sample with endo- or exopeptidases may

cause rapid sample degradation.

IV. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE

WATERS PROTEIN EXPRESSION SYSTEM USING NANOSCALE (75

m scale) HPLC

One vial of MPDS E. coli is required to execute the Waters Protein

Expression System training module. MPDS E. coli is prepared by resus-

pension of the lyophilized powder in 250 µL of 0.1% formic acid. Mix

capped vial by vortexing to insure all material is resuspended. Refer to

the Waters Protein Expression System Operator’s Guide for details on

the use of the MPDS E. coli mixture for system training purposes.

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[ Care and Use ManUal ] 1

Sommario

Pagina 1 - [ Care and Use ManUal ]

E. Coli Digestion Standard 1[ Care and Use ManUal ]CONTENTSI. INTRODUCTIONII. VIAL CONTENTSIII. STORAGE AND STABILITYIV. RECONSTITUTION OF MPD

Pagina 2

E. Coli Digestion Standard 2[ Care and Use ManUal ]V. RECONSTITUTION OF MPDS E. COLI FOR ANALYSIS WITH THE WATERS PROTEIN EXPRESSION SYSTEM USING MICR

Pagina 3

E. Coli Digestion Standard 3[ Care and Use ManUal ]Sales Offices:Austria and European Export (Central South Eastern Europe, CIS and Middle East) 431 8

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