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[ Care and Use ManUal ]

Oasis HLB Glass Cartridges 1

Contents

I. IntroduCtIon

II. QuICk start sPe ProCedure

III. adjustments to oPtImIze reCoverIes

Iv. PreParatIon of PhosPhate-Buffered salIne (PBs)

a. Sample Comminution

b. Extraction/Partitioning

c. Dispersive Solid-Phase Extraction

v. orderIng InformatIon

a. Extraction Solution Preparation

oasIs hlB glass CartrIdges

I. IntroduCtIon

Waters Oasis

HLB glass cartridges are available in 5 cc (200 mg)

configuration with Teflon

frits. The clean glass cartridge is designed

for trace analysis at parts per trillion level including monitoring

endocrine disruptors, such as phenols and phthalates. The Oasis

glass cartridge contains a unique (patented

) sorbent, a copolymer

designed to have a hydrophilic-lipophilic balance (HLB), that gives

high and reproducible recoveries for acidic, basic, and neutral com-

pounds - even if the cartridge runs dry.

E a c h l o t o f g l a s s c a r t r i d g e s a n d T e f l o n f r i t s a r e t e s t e d f o r t h e

presence of bisphenol A and other phenols and phthalates before

packing. These tests assure that endocrine disruptors, in water

samples, can be analyzed to part per trillion levels.

This Instruction Sheet outlines in Section II the Quick Start Solid-

Phase Extraction (SPE) Procedure for extracting endocrine disruptors

and many other types of compounds. Section III gives a simple

protocol to determine if any adjustments are necessary to optimize

recovery. Section IV describes the preparation of phosphate-buffered

saline (PBS) solution (required only for serum, plasma or urine

matrix).

The Certificate of Analysis (COA) reports recoveries, with RSDs, for

three polar pharmaceutical compounds. The COA displays results

from stringent quality control tests on the batch of polymer sorbent

and the lot of packed cartridges.

US Patent # 5,882,521

1 2 3

[ Care and Use ManUal ] 1

Sommario

Pagina 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]Oasis HLB Glass Cartridges 1ContentsI. IntroduCtIonII. QuICk start sPe ProCedureIII. adjustments to oPtImIze reCoverIesI

Pagina 2

[ Care and Use ManUal ]Oasis HLB Glass Cartridges 2II. QuICk start sPe ProCedureI. If desired, add 10 to 50 μL of internal standard to the sample (s

Pagina 3

[ Care and Use ManUal ]Oasis HLB Glass Cartridges 3Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www

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