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[ Care and Use ManUal ]

1Sirocco

Protein Precipitation Plate

Sirocco Protein PreciPitation Plate

The Sirocco

™

plate was designed to improve the automation and through-

put of protein precipitation in drug metabolism/ADME/toxicology labs.

The Sirocco Protein Precipitation Plate is a unique combination of novel

96-well plate tip design with proprietary new filter membranes.

A straightforward 7-step procedure designed to enable precipitate free

sample filtrates suitable for high-throughput LC/MS analysis.

Step 1 – Preparation

Place the Sirocco Plate on a collection plate for processing. This protects

the valve tips during the processing steps.

Sirocco Protein

Precipitation Plate

96-Well Collection Plate

Step 2 – Add Crash Solvent

Add precipitation solvent to all wells. Use a ratio of at least 3:1 solvent

to sample. Acetonitrile is the recommended precipitation solvent. The

Sirocco plate is designed to prevent solvent from leaking through the

plate at this step.

Step 3 – Load Sample

Add 20-200 µL of sample (plasma, urine, etc.) rapidly into the wells. Rapid

delivery of sample into the crash solvent creates mixing and initiates

precipitation process.

1 2 3

Sommario

Pagina 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]1Sirocco Protein Precipitation PlateSirocco Protein PreciPitation PlateThe Sirocco™ plate was designed to improve the automatio

Pagina 2

[ Care and Use ManUal ]2Sirocco Protein Precipitation PlateStep 4 – SealApply vented cap mat, using a cap roller to ensure secure, uniform sealing. Th

Pagina 3 - Sales Offices

[ Care and Use ManUal ]3ORDERING INFORMATIONDescription Volume Qty/Pkg Part NumberSirocco Protein Precipitation Plate (includes 1 plate and 1 cap mat)

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