Waters IC-Pak C Method Development Column Manuale Utente Scaricare il pdf

[ Care and Use ManUal ]

Waters IC-Pak C M/D Column 1

Contents

I. IntroduCtIon

a. Waters IC-Pak C M/D Column Description

b. Specifications

II. InstALLInG tHe CoLuMn

a. Preparing the Ion Exchange System

b. Installing the Column

c. Equilibrating the Column

III. PrePArInG MoBILe PHAse And sAMPLes

a. Preparing the Mobile Phase

b. Preparing the Sample Preparing

IV. usInG tHe CoLuMn

a. Chromatography Guidelines

b. Efficiency Testing

V. CAre & MAIntenAnCe

a. Storing the Column

b. Troubleshooting

VI. orderInG InForMAtIon

WAters IC-PAk C M/d CoLuMn

I. IntroduCtIon

This manual describes procedures for using the Waters IC-Pak™ C M/D

Ion Chromatography separation of monovalent and divalent cations.

Please take a few moments to read this manual carefully. Follow its

recommendations to obtain reproducible chromatography and column

stability.

a. Waters IC-Pak C M/D Column Description

Waters IC-Pak C M/D column is designed for ion chromatography

applications. Use the M/D column to simultaneously analyze

monovalent and divalent cations isocratically. The column separates

and allows quantitation of Li

, Na

, NH

*, Mg

’ Ca

, Sr

, and Ba

cations at ppb levels.

b. Specifications

Column IC-Pak C M/D

Dimensions 3.9 x 150 mm

Particle size 5 µm

Capacity 1.5 ± 0.2 meq/g

Packing material Silica base coated with polybutadi-

ene/maleic acid copolymer

Shipped in 20/80 (v/v} acetonitrile/water

1 2 3 4 5 6

Sommario

Pagina 1 - WAters IC-PAk C M/d CoLuMn

[ Care and Use ManUal ]Waters IC-Pak C M/D Column 1ContentsI. IntroduCtIona. Waters IC-Pak C M/D Column Descriptionb. SpecificationsII. InstALLIn

Pagina 2

[ Care and Use ManUal ]Waters IC-Pak C M/D Column 2II. InstALLInG tHe CoLuMna. Preparing the Ion-Exchange SystemTo prepare the system, you need two 3/

Pagina 3

[ Care and Use ManUal ]Waters IC-Pak C M/D Column 3FiltrationBefore filtering the mobile phase, flush the filters with 100 mL of mobile phase. Discard

Pagina 4

[ Care and Use ManUal ]Waters IC-Pak C M/D Column 4General considerations• Filterallaqueousbuffers.Donotuseturbidorcloudybuffers.• Donot

Pagina 5

[ Care and Use ManUal ]Waters IC-Pak C M/D Column 5To prepare 1 liter of working standard:1. Measure the appropriate quantity of stock standard (refe

Pagina 6

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comWaters IC-Pak C M

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