Waters ACQUITY UPLC HSS Columns Manuale Utente

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[ Care and Use ManUal ]
XSELECT HSS HPLC COLUMNS
1
Contents
I. GettInG started
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard
Pre-Columns
II. Column use
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III. Column CleanInG, reGeneratInG and storaGe
a. Cleaning and Regeneration
b. Storage
IV. IntroduCInG ECord IntellIGent ChIp
teChnoloGy
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
V. addItIonal InformatIon
a. Tips for Maximizing ACQUITY UPLC HSS
Column Lifetimes
b. Recommended Flow Rates and Backpressures
for Reversed-Phase ACQUITY UPLC HSS Columns
Thank you for choosing a Waters ACQUITY UPLC
®
HSS column. The
ACQUITY UPLC HSS packing materials were designed specifically
for use with the Waters ACQUITY UPLC system and are manufactured
in a cGMP, ISO 9001:2000 certified plant using ultra pure reagents.
Each batch of ACQUITY UPLC HSS material is tested chromato-
graphically with acidic, basic and neutral analytes and the results
are held to narrow specification ranges to assure excellent, repro-
ducible performance. Every column is individually tested and a
Performance Chromatogram and Certificate of Batch Analysis are
provided on the eCord
intelligent chip.
ACQUITY UPLC columns were designed and tested specifically for
use on the ACQUITY UPLC system. ACQUITY UPLC columns will
exhibit maximum chromatographic performance and benefits ONLY
when used on the holistically-designed ACQUITY UPLC system since
the ACQUITY UPLC system and column were created and designed
to operate together. For these reasons, Waters cannot support
the use of ACQUITY UPLC columns on any system other than an
ACQUITY UPLC system.
aCQuIty uplC hss Columns
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Sommario

Pagina 1 - [ Care and Use ManUal ]

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS1ContentsI. GettInG starteda. Column Connectors b. Column Installation c. Column Equilibration d.

Pagina 2

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS2I. GettInG startedEach ACQUITY UPLC HSS column comes with a Certificate of Analysis and a Performance

Pagina 3

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS3e. Initial Column Efficiency Determination 1. Perform an efficiency test on the column before using i

Pagina 4

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS4and 1 to 8 for the HSS C18 chemistry. A listing of commonly used buffers and additives is given in Tab

Pagina 5

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS5Note: Working at the extremes of temperature, pressure and/or pH will result in shorter column lifetim

Pagina 6

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS6d. Storage post Normal-Phase useFor rapid equilibration upon start-up, it is recommended that you stor

Pagina 7

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS7d. Column Use InformationThe eCord chip provides the customer with column use data. The top of the scr

Pagina 8

[ Care and Use ManUal ]XSELECT HSS HPLC COLUMNS8b. Recommended Flow Rates and Backpressures for Reversed-Phase ACQUITY UPLC HSS Columns1.0 mm i.d. Col

Pagina 9 - Sales Offices

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comNAME OF DOCUMENT9

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