Waters Oligonucleotide Separation Technology ACQUITY UPLC Manuale Utente

[ method guidelines ]ACQUITY UPLC® OST C18 columns are based on Waters second gen-eration of hybrid-silica BEH Technology™ particles and can be effec-

2[ method guidelines ]I. P rInC IPLes oF oLIgonUCLeotIde se PA rAtIons Separations of detritylated synthetic oligonucleotides on ACQUITY UPLC®

3[ method guidelines ]3. To remove particulates the sample may be filtered with a 0.2 μm membrane. Be sure that the selected membrane is compatible an

4[ method guidelines ]Mobile Phase B change per min) is required. In these situations, it is recommended that the organic solvent concentration in Mob

5[ method guidelines ]enhanced component resolution. A suitable gradient separation method can be approximated from the oligonucleotide base (C, G

6[ method guidelines ]1: Standard oligonucleotides: 10 – 30 mers 2: Short oligonucleotides: Less than 10 mer 3: Long oligonucleotides: 30 – 60 mers

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com[ method guidelines ]©2007 Waters Corpor